We recommend using a heat seal (rather than adhesive) or strip caps on top of the plate.Plates must sealed extremely thoroughly to survive shipping without leaking - sadly we receive a lot of leaky plates.Place the wrapped tubes into a small protective vessel (such as a Falcon tube or small box).Wrap caps in one light layer of parafilm to keep the caps closed.Ensure caps fit snugly and close them firmly.If sending PCR strip tubes or single PCR tubes: Improperly labeled samples may be discarded.
If you’re sending a single sample in a single PCR tube, label with the 3-digit order label only.ĭO NOT label your tubes with sample names, sample numbers, or any other info! as demonstrated by the scary dinosaurs in the photo below:
Write the strip number on the 2nd tube of each strip. Write your order label on the 1st tube of each strip. Other type of tubes are not compatible with our rapid workflow and may be discarded.Įach order will be assigned a 3-character order label after the order has been confirmed through Samples should ONLY be sent in PCR strip tubes (or single PCR tubes if shipping single The volume listed is the minimum required, but it never hurts to send more in case some is lost to evaporation. Our protocols have been shown to be tolerant to EDTA, but we recommend avoiding it if possible just in case. low purity, excess genomic DNA, excess RNA, degraded plasmids) will not yield satisfactory data and may result in sequencing failure. The target plasmid (or amplicon) needs to be the dominant species in the sample to produce high quality sequencing results. Unlike Sanger sequencing, which uses a primer to target specific sequences, we sequence everything in the sample. The extent to which Nanodrop is off varies widely depending on the specific composition of your sample, so there is no standard way to“adjust” Nanodrop values. Sending samples with concentrations that are too high or too low will adversely affect the library prep and/or sequencing reactions, possibly resulting in sequencing failure. Please match your sample concentration as closely as possible to the required concentration. Nanodrop is NOT ADEQUATE due to low accuracy. We strongly recommend using Qubit or equivalent to measure DNA concentration. Required concentration and volume listed below. We keep our prices low and turnaround times fast by trusting our customers to provide high-quality DNA.
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